crna cy3 fluorescence labeling Search Results


streptavidin cy3  (Cytiva Europe)
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Cytiva Europe streptavidin cy3
Streptavidin Cy3, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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arraystar epitranscriptomics microarray  (Arraystar inc)
90
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Arraystar Epitranscriptomics Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crna  (Cytiva Europe)
93
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Crna, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human mrna&lncrna epitranscriptomic arrays (8 × 60 k)  (Arraystar inc)
90
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Human Mrna&Lncrna Epitranscriptomic Arrays (8 × 60 K), supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cy3 fluorescent crna  (Arraystar inc)
90
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CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with <t>Cy3-labeled</t> circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Cy3 Fluorescent Crna, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crna+cy3+fluorescence+labeling/cy5/pmc08446459-54-8-16
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cy3 mono reactive dye  (Cytiva Europe)
95
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CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with <t>Cy3-labeled</t> circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Cy3 Mono Reactive Dye, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rneasy mini kit  (Qiagen)
99
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CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with <t>Cy3-labeled</t> circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crna cy3 fluorescence labeling  (Cytiva Europe)
96
Cytiva Europe crna cy3 fluorescence labeling
CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with <t>Cy3-labeled</t> circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Crna Cy3 Fluorescence Labeling, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crna+cy3+fluorescence+labeling/Cy3+NHS+Ester/10__1016_slash_j__proenv__2011__10__104-46-23-27
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crna cy3 fluorescence labeling - by Bioz Stars, 2026-09
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super rna labeling kit  (Arraystar inc)
90
Arraystar inc super rna labeling kit
CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with <t>Cy3-labeled</t> circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Super Rna Labeling Kit, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyanine 3 ctp cy3  (Revvity)
91
Revvity cyanine 3 ctp cy3
CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with <t>Cy3-labeled</t> circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Cyanine 3 Ctp Cy3, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rneasy kit  (Qiagen)
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CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with <t>Cy3-labeled</t> circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crna+cy3+fluorescence+labeling/RNeasy+FFPE+Kit/pmc08267322-140-8-7
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fluorescent crnas combined cy5 with cy3  (Arraystar inc)
90
Arraystar inc fluorescent crnas combined cy5 with cy3
CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with <t>Cy3-labeled</t> circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Fluorescent Crnas Combined Cy5 With Cy3, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with Cy3-labeled circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Journal: Frontiers in Cell and Developmental Biology

Article Title: CircGLIS3 Promotes High-Grade Glioma Invasion via Modulating Ezrin Phosphorylation

doi: 10.3389/fcell.2021.663207

Figure Lengend Snippet: CircGLIS3 directly binds with p-Ezrin(T567) and elevates the p-Ezrin(T567) level in glioma. (A) Fluorescence in situ hybridization (FISH) assay with Cy3-labeled circGLIS3 probe (magnification, ×640; scale bar, 10 μm). (B) Western blot of GLIS3 protein after the upregulated or downregulated circGLIS3 level in U251 cells. (C) RNA-Seq analysis of U251 cells transfected by sh-circGLIS3 or sh-NC lentivirus. (D) KEGG and (E) GO enrichment analysis in U251 cells. (F) RNA pull-down experiments with U251 extract. Pull-down probe efficiency was determined by RT-PCR. (G) RNA pull-down experiments with U251 extract. Specific bands were identified by mass spectrometer. (H) Summary of circGLIS3 binding proteins according to KEGG. (I) Western blot detection after upregulated or downregulated circGLIS3 in U251. (J) Western blot detection of p-Ezrin(T567) in U87 transfected by concentration gradient circGLIS3 plasmid. (K) Western blot of RIP lysate by anti-Ezrin, anti-p-Ezrin(T567), anti-IgG, and anti-SNRNP70 antibodies. (L) RT-PCR analysis of Ezrin, p-Ezrin(T567), IgG-negative control, and SNRNP70-positive control immunoprecipitated RNA of U87 (left) and U251 (right). (M) Western blot of p-Ezrin(T567) in U87 and U251 after being treated with DMSO or fasudil hydrochloride for 2 days. (N) Western blot of U87 and U251 after treated with GO6983 for 2 days. (O) RT-PCR of U87 and U251 after being treated with GO6983 for 2 days. (P) Confocal microscope image of Ezrin and ROCK2 or RIOK1. Scale bar, 5 μm. (Q) Western blot of p-Ezrin(T567) in U87 after being treated with NSC305787 for 9 h. The results are presented as the mean ± SEM of biological triplicate assays. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: The enriched circRNAs were amplified and transcribed into Cy3 fluorescent cRNA utilizing a random priming method (Arraystar Super RNA Labeling Kit; Arraystar).

Techniques: Fluorescence, In Situ Hybridization, Labeling, Western Blot, RNA Sequencing, Transfection, Reverse Transcription Polymerase Chain Reaction, Mass Spectrometry, Binding Assay, Concentration Assay, Plasmid Preparation, Negative Control, Positive Control, Immunoprecipitation, Microscopy